Dear Amr,
the gel is some kind of phase separation enriched in your protein
(perhaps indeed aggregates).
The fact that both the crystals and the "gel" gives blue tryptophan
fluorescence clearly shows that both are protein.
I think it is quite frequent that these kind of phase separations serve
as nucleation points.
Well done, shoot'em!
Matthias
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Dr. Matthias Zebisch
Division of Structural Biology,
Wellcome Trust Centre for Human Genetics,
University of Oxford,
Roosevelt Drive,
Oxford OX3 7BN, UK
Phone (+44) 1865 287549;
Fax (+44) 1865 287547
Email matth...@strubi.ox.ac.uk
Website http://www.strubi.ox.ac.uk
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On 10/19/2013 7:24 PM, amro selem wrote:
Dear Cryptographers,
I have repeated question , i did screening and with in one day i got crystals,
also this is some gelled protein . under UV, the crystals appear blue but
also the gelatin like precipitate too . i will attach the photo for some help
if the birefrigence say some information if it is salt or protein. the screen
condition is 0.2 ammonium sulphate and 15% peg 8000.also have feeling that
crystals are still growing .
best regards
Amr
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