Hi, If there is more than one strong unique Patterson peak, then Phaser currently doesn't know how to interpret the tNCS. If all the peaks can be interpreted as multiples of one one vector (taking symmetry into account) then you can use the NMOL argument of the tNCS command. Otherwise, you can get Phaser to only take account of the top peak by setting the minimum peak height.
If you post or email the list of peaks, I might be able to give some specific advice (although I'll be on my way to the Cold Spring Harbor school most of the day). Best wishes, Randy Read ---- Randy J. Read > On 22 Oct 2013, at 23:35, Niu Tou <[email protected]> wrote: > > Dear All, > > I have a MR case which may contain two different molecules, ideally 1:1 > ratio. Solvent content analysis indicates most likely to be 4~6 hetero > molecules. The data shows at least 5 strong translational NCS vectors. > > When I tried to run PHASER, it always said "correction factors NOT applied", > but I have chosen to count it in the interface. Is there anyway I could use > these NCS information, either by checking some options in the GUI of > modifying the script? Many thanks! > > Best, > Niu
