Just a quick off-topic question which people here may have experience in: We have a protein which expresses with a GST tag. It has much poorer expression without. When the tag is cleaved, the protein does not crash out but we are having difficulty separating it as the proteins seem to stick together. When run on a GST column, some of the protein elutes with GST and some sticks to the column with GST. We are losing over 90% in this manner. The protein expresses to ~2mg/ml but is relatively stable and has crystallized. If anyone has similar experiences that they managed to resolve(or didn't) or any suggestions, I would appreciate it.
Jon Read Associate Principal Scientist _____________________________________________________________________ Astrazeneca R&D, Decs, Astrazeneca, Mereside, Alderley Park, Cheshire, SK10 4TG UK Tel +44 (0)1625 510661 Mobile +44 (0)7775 040376 -------------------------------------------------------------------------- AstraZeneca UK Limited is a company incorporated in England and Wales with registered number: 03674842 and a registered office at 2 Kingdom Street, London, W2 6BD. Confidentiality Notice: This message is private and may contain confidential, proprietary and legally privileged information. If you have received this message in error, please notify us and remove it from your system and note that you must not copy, distribute or take any action in reliance on it. Any unauthorised use or disclosure of the contents of this message is not permitted and may be unlawful. Disclaimer: Email messages may be subject to delays, interception, non-delivery and unauthorised alterations. Therefore, information expressed in this message is not given or endorsed by AstraZeneca UK Limited unless otherwise notified by an authorised representative independent of this message. No contractual relationship is created by this message by any person unless specifically indicated by agreement in writing other than email. Monitoring: AstraZeneca UK Limited may monitor email traffic data and content for the purposes of the prevention and detection of crime, ensuring the security of our computer systems and checking Compliance with our Code of Conduct and Policies.
