Dear all, The gly104 sequence is correct. This is the substrate complex structure. In case of native structure, such strange of blob1 didnot appear Also for the second blob, I follow your suggestion and the result is nice I am still waitting for the suggestion of the first blob
Thank you a lot, Sincerely yours On Thursday, October 31, 2013 4:45 PM, Prof. Dr. Arne Skerra <[email protected]> wrote: Can you exclude a sequencing error for Gly104? Am 31.10.2013 um 08:40 schrieb thuy ngo: Dear all, > > >I am dealing with two strange extra density map. One is connected from Cb of >Proline to Ca of glycine (blob 1) > and other is positioned between two symmetric molecules which is connected >from Cb of Histidine to carboxylate group of Glutamate. I checked all the >crystallization and purification condition but no ligand is suitable for these >blobs. Could you suggest some possible candidates? >This is the structure complex between peptide deformylase and fMAS >Crystallization condition: 2M Sodium acetate, 0.1 M HEPES 7.5 and 0.05M CdSO4 > > >Thank you<blob1_back.png><blob1_front.png><blob2.png> Prof. Dr. Arne Skerra Lehrstuhl f. Biologische Chemie | Technische Universitaet Muenchen Emil-Erlenmeyer-Forum 5 | 85350 Freising-Weihenstephan | Germany Phone: +49 (0)8161 71-4351 | Fax: -4352 eMail: [email protected] | http://www.wzw.tum.de/bc
