Thank you very much for kind suggestions and comment. With kind regards Prem
On Mon, Dec 2, 2013 at 5:41 PM, Randy Read <rj...@cam.ac.uk> wrote: > Hi, > > If there is a hinge motion between the two domains, then allowing for this > will give you a much better starting model. As Klaus suggested, you may > just be able to do rigid body refinement of the two domains. However, it > is also possible to place the two domains separately in Phaser, by putting > the two domains in separate PDB files, defining an ENSEMBLE with each of > these PDB files, then searching for both of them in the same job. In the > ccp4i GUI, you can specify an additional ensemble by clicking the Add > ensemble button, and you can add another component to search for in the > same job by clicking the Add another search button. > > I hope that answers the question you were asking! > > Best wishes, > > Randy Read > > On 2 Dec 2013, at 04:11, Prem Prakash <prem...@gmail.com> wrote: > > > Dear All, > > > > The density obtained after molecular replacement using phaser at 2.5 > Angstrom and then used buccneer for autobuilding of the model. I am not > getting reasonable R value (it is 38.5 %) but the figure of merit is 0.629. > > > > As My protein has two domains. So is it possible to fragment the > individual domain and then again perform the molecular replacement. How it > will improve my phase more and how R-factor will be reduced ? > > > > Please help and direct me in proceeding in a right way, and if possible > provide a protocol for doing that. Thank you > > > > With kind regards > > > > > > ------ > Randy J. Read > Department of Haematology, University of Cambridge > Cambridge Institute for Medical Research Tel: + 44 1223 336500 > Wellcome Trust/MRC Building Fax: + 44 1223 336827 > Hills Road E-mail: rj...@cam.ac.uk > Cambridge CB2 0XY, U.K. www-structmed.cimr.cam.ac.uk > >
