Hi, I would only suggest using the brute rotation function if you want to use the "rotate around" option or do something other than a full rotation search, because the fast search followed by rescoring should give you essentially the same list of peaks (but faster).
If you want to get a bigger list of orientations, you should be able to open the "Expert parameters" section of the ccp4i GUI for Phaser, then change the percent cutoff in the "Purge rotation peaks" line to something lower than the default of 75%. Alternatively, if you're running Phaser from a script, you can include commands like: PURGE ROT ENABLE ON PURGE ROT PERCENT 50.0 I've just tested and this works for me. Let me know if you're still having trouble with this. Best wishes, Randy Read On 5 Dec 2013, at 10:11, LISA <[email protected]> wrote: > hi all, > > I try to solve the crystal structures of a mult-domain protein with phaser. I > cannot solve the structure by automatic search using domain structure as the > models. I tried to do the rotation search using the fast or brute rotation > function. I defined the rotation search peaks number is 1000. But I only get > no more than 100 peaks. I tried the phaser in ccp4-6.3.0 and ccp4-6.4.0. But > I can only get much less peaks than I required in the rotation. But the old > version phaser can give about 1000 peaks if I use brute rotation function ask > for 1000 peaks. How can I more rotation peaks in the new version phaser > ? > Thank you. > > Lisa ------ Randy J. Read Department of Haematology, University of Cambridge Cambridge Institute for Medical Research Tel: + 44 1223 336500 Wellcome Trust/MRC Building Fax: + 44 1223 336827 Hills Road E-mail: [email protected] Cambridge CB2 0XY, U.K. www-structmed.cimr.cam.ac.uk
