Hi, I am facing a problem in refining one of my structures. With every refinement the Rwork is going down. However, the Rfree is stuck and is not going down with refinement. I have already solved the structure without the co-factor bound. This structure is with the co-factor bound. Can anyone suggest the reason for the Rfree getting stuck?
Thanks -- Nazia Nasir PhD Scholar Protein Crystallography Lab National Institute of Immunology New Delhi
