Dear Afshan,
Maybe what I suggest you have already tried: have you tried to fit a
citrate molecule? The blob seems to be branched, thus compatible with
such organic molecule.
Danilo
On Thu, 12 Dec 2013 03:34:07 -0800, Afshan Begum <[email protected]>
wrote:
Dear Experts,
I collected a data set (1.12A) of one of my target proteins, and
built
a model by molecular replacement.
I then exam the model by Coot and found one unidentified blob. I am
unable to to identified a blob which appeared on the surface of the
molecule
its close to residues 115 SER
the protein purified in 10 mM Na-phosphate buffer, 100mM KCl and
crystallized in 1.6 M sodium citrate, 3.5% _v/v _glycerol
I have attached the picture of the blob.
I would be thankful for your kind suggestions.
Best Regards
AFSHAN
