The kinase domain of FAK can be made constitutively active by a mutation and expressed in E. coli. It's called "Super FAK Kinase". But i am afraid it is rather specific. If you don't get a better lead I can send you the sequence and a reference.
Gloria Borgstahl wrote:
Does anyone know of a good way to phosphorylate the Tyr on a protein for structural studies? Is there a generic kinase that can be coexpressed or purified for phosphorylation? The pCMF Amber codon system is very expensive and Glu really doesn't mimic pTyr all that well. Any ideas/help would be appreciated, G
