You can try MST(MicroScale Thermophoresis), if there is an instrument available. It is like a mini ITC, but much more versatile. The sample consumption is very low, claimed by the company that sells the instrument. http://www.nanotemper-technologies.com/technology/microscale-thermophoresis/article/key-features/ It is possible to ask the company to perform a demo and test some samples in your lab.
Good luck, Jia-Ying On 13 January 2014 14:47, George Kontopidis <[email protected]> wrote: > *Specifically for fluorescence* > > does your ligand fluoresce? > > It is possible if it has indol group or some aromatic organic compound > > > > Does your protein has a tryptophan or tyrosines in the binding site? > > If yes may be a fluorescence titration experiment could be the solution. > > > > Also fluorescence needs very low concentration of protein (nM to microM) > > > > george > > > > > > *From:* CCP4 bulletin board [mailto:[email protected]] *On Behalf Of > *Acoot > Brett > *Sent:* Monday, January 13, 2014 3:09 PM > *To:* [email protected] > *Subject:* Re: [ccp4bb] Assays for protein-ligand interaction? > > > > FRET， CD， Fluorescence， NMR chemical shift assay, isotope-labelled ligand > interaction assay, protein melting temperature assay, gel filtration > retention assay, gyration radius assay by Malls, native page gel analysis, > etc. > > > > Acoot > > > > On Monday, 13 January 2014 8:51 PM, HJ Lee <[email protected]> wrote: > > Sorry for the off topic. I'm looking for a way to monitor > protein-(potential) ligand interaction. The ligand is small molecule > (mw~250) and we're looking for its potential interaction with couple human > proteins. (We do not know this small molecule interacts with these human > protein or not.) > > > > Is there any efficient way to quickly identify whether this ligand > interacts with those human protein? We can buy some protein, but the amount > of commercially available purified proteins is very little, making them > hard to be analyzed by some good methods (e.g. ITC). > > > > That would be really great if anyone suggest any idea. Sorry for the off > topic question again. > > > > Thanks! > > >
