-----BEGIN PGP SIGNED MESSAGE----- Hash: SHA1 Dear Klaus,
this list seems to be missing means to reduce the growth rate, in particular micro seeding applied to a matrix screen of protein vs. precipitant concentration: Find the condition(s) just below crystals form and micro-seed with a cat whisker! Best, Tim On 01/22/2014 04:01 PM, Klaus Ftterer wrote: > Dear ccp4bb contributors, > > We are dealing with the problem of a protein (~ 50 kDa) that > crystallises readily, but has an annoying habit of forming highly > intergrown rods or needles. Even when the crystals look optically > homogenous under the microcsope, diffraction is so so (3.5 ￅ or so > on the synchrotron), but patterns reflect several crystal lattices > that the processing software cannot resolve properly. > > We have tried this: > > - additive screens - switching the His-tag from N- to C-terminus - > cutting the tag - thermal stability screens in a variety of > buffers - growth in the presence of potential ligands/substrates > > Any suggestions for tricks that we haven't thought of so far? > > Thank you. > > Klaus > > > ======================================================================= > > Dr. Klaus Ftterer > > School of Biosciences P: +44-(0)-121-414 5895 University of > Birmingham F: +44-(0)-121-414 5925 Edgbaston > E: [email protected] Birmingham, B15 2TT, UK W: > http://tinyurl.com/futterer-lab > ======================================================================= > > > > > > > - -- - -- Dr Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen GPG Key ID = A46BEE1A -----BEGIN PGP SIGNATURE----- Version: GnuPG v1.4.12 (GNU/Linux) Comment: Using GnuPG with Icedove - http://www.enigmail.net/ iD8DBQFS399XUxlJ7aRr7hoRAq3EAKDH+qffLg3/+XuxykVV8U4WedoBHwCfZt1z TYV/SK+44hAcniVpfLQKxeY= =k/0t -----END PGP SIGNATURE-----
