Dear Lisa, because we know nothing about our object: protein is membrane
or globular? Is there known structures of homologs?
What buffer do you use?
17.02.2014 13:57, LISA пишет:
Dear All,
My proein is polymerzated and elutate in the void volum when runnning
gel filtration Superdex 200. I can get a small crystal after a lot of
optimization. But the resolution is still very low (about 8A ). I try
to find the sites involved in polymerization. Is there some software
to predicat the amino acids which are involved in protein
polymerization? And All suggestion to the crystal optimization are
welcome. Thank you.
Best Regards,
Lisa
--
Eugene Osipov
Junior Research Scientist
Laboratory of Enzyme Engineering
A.N. Bach Institute of Biochemistry
Russian Academy of Sciences
Leninsky pr. 33, 119071 Moscow, Russia
e-mail: [email protected]
