Hi, what was not mentioned in the ccp4 discussion is
The use of trimethylamine N-oxide as a primary precipitating agent and related methylamine osmolytes as cryoprotective agents for macromolecular crystallography Acta Cryst. (2012). D68, 69-81 I found it as a very potent compound for cryo-preservation of crystals. Kind regards, Reiner -- Dr. Reiner Kiefersauer research scientist Proteros Biostructures GmbH Bunsenstraße 7a D-82152 Planegg-Martinsried Germany Tel.: +49 (0) 89 700761 -0 Fax: +49 (0) 89 700761 -15 E-mail: kiefersa...@proteros.de<mailto:kiefersa...@proteros.de> Die in dieser e-mail beinhaltete Information unterliegt der Geheimhaltung. Sie ist ausschließlich für den genannten Empfänger bestimmt. Falls Sie nicht der genannte Empfänger sind, bitten wir Sie, uns darüber in Kenntnis zu setzen und alle bestehenden Kopien zu vernichten. This message and any attached files contain privileged and/or confidential information intended only for the use of the addressee. If you are not the addressee, or the person responsible for delivering it to the person addressed, you may not read, use, copy or deliver this to anyone else. If you received this message by mistake, please immediately notify us and delete it from your system. All care has to be taken to ensure this message and any attachments are virus free. No responsibility is accepted for any virus infections caused by the receipt of this message. Geschäftsführer: Dr. Torsten Neuefeind, Arnd Christ Amtsgericht München HRB-Nr. 121 476 - VAT-ID: DE 195 857 418 - Steuernummer 143/173/20265