Dear Timothy, My is non-membrane protein. The proteins you recommended are also suitable, right? Cheera, Acoot
On Thursday, 27 February 2014 7:07 PM, Timothy Craig <[email protected]> wrote: You may want to try cytochrome B 562 RIL (BRIL), T4 Lysozyme, rubredoxin, or amicyanin. These are commonly used for crystallizing difficult membrane proteins. The protein sequences can be found in the PDB. You can get the DNA from any gene synthesis vendor like dna2.0, geneart, or genscript. -Tim Craig ________________________________ Date: Thu, 27 Feb 2014 02:29:58 -0800 From: [email protected] Subject: [ccp4bb] protein domain for crystalization contact To: [email protected] Dear All, After series of trial and even with the truncated forms, I find my protein has the difficulty to contact to form the crystallization, or lacking the nucleation center in the purified protein. Will you please introduce the domains with witch my protein fused, there will be more contact points for the crystallization formation, besides the traditionally known Fab fragment? Cheers, Acoot
