Hi Amlan, given that it should not depend on which school of thought you follow: 2*(Fo-Fc) + Fc vs Fo + (Fo-Fc), you should be able to see the same thing at appropriately chosen contouring levels in both maps, I think... (I could be wrong at this time of the night, of course!)
Pavel On Mon, Mar 10, 2014 at 2:09 AM, Amlan Roychowdhury <[email protected]>wrote: > Dear All, > > Some times during model building in coot we have found that at the > position of ligand molecules and water, there is a good Fo-Fc map (above 3 > sigma), devoid of any 2Fo-Fc map. > > 1.What does it physically mean and why the 2Fo-Fc map was not generated > properly? > > 2. Can we fit ligand molecule there? > > Thanks in advance. > > Best Wishes > Amlan. > > -- > Amlan Roychowdhury. > Senior Research Fellow. > Protein Crystallography Lab. > Dept. of Biotechnology, > IIT Kharagpur. > Kharagpur 721302 > West Bengal. > India. >
