Dear Friends
I have a protein-peptide complex crystals, which is diffracted at 3.8 resolution with a P41212 space group. We solved the structure using molecular replacement method, which shows that there are thre molecules in asymmetric unit. Unfortunately one of the chain in this asymmetric unit is poorly defined and then I attempted density averaging using Density modification program from CCP4 suite. It is fained with error massage: *dm:(RCARDS) SOLC:content missing or out of range* The solvent content is around 75 percent and I have submitted a recent refmac mtz file for the job with rotation and translation matrix ( to have an idea anout NCS). I have also checked 'Input starting map coefficients'. Can any one help in finding what is the problem and how to sort it out? Thanking you in advance. DCB -- Mr.Dilip C. Badgujar, Senior Research Fellow, ACTREC, Tata Memorial Center, Sector-22, Kharghar, Navi Mumbai. Pin-410210
