Dear All, I want to get some help regarding crystallization for one of the protein I am working. This is a recombinant protein of molecular weight of 17.5 kDa. I am getting crystals shape like thin plates in 5 days, in different conditions like bis tris pH 6.5-8, HEPES pH 6-8 with .1M nacl , .01M DTT having PEG 3350, PEG 6000 as precipitant. But whenever we used to put crystal in cryoprotectant like 20 % ethylene glycol, glycerol, MPD it used to split like layers of some tree barks. And the crystal which were diffracting not getting diffracted above 3.0 Angstrom.
Please help me out. Thanks in advance -- Anamika
