Hi Xiaoming, I would download pdb2pqr 1.8 from the apbs website and install it. (linux) It can handle nucleic acid, (3 letter code) . After you installed it run it with python /whereeveritis/pdb2pqr-1.8/pdb2pqr.py -h
it will print the options. Hope this helps. Fred [32m******************************************************************************* Fred Dyda, Ph.D. Phone:301-402-4496 Laboratory of Molecular Biology Fax: 301-496-0201 DHHS/NIH/NIDDK e-mail:fred.d...@nih.gov Bldg. 5. Room 303 Bethesda, MD 20892-0560 URGENT message e-mail: 2022476...@mms.att.net Google maps coords: 39.000597, -77.102102 http://www2.niddk.nih.gov/NIDDKLabs/IntramuralFaculty/DydaFred *******************************************************************************[m