Dear colleagues, I know this is not related to ccp4 but I am in need of an answer and many of you work with cells etc.
My building had a major malfunction of electricity and the power backup did not kick in. My -80C freezer was without power for over 24 hours until I found out. Because it is small, it goes fast to room temp. I had many glycerol stocks in it with cells, cells with plasmids etc. as well as cell pellets. I am trying to rescue things. My question is what happens to cell pellets. I had many as I like to start purification at the cell pellet level. Are these destroyed when they go to room temp for 24 hours or are they ok? Thanks. Jackie Vitali Cleveland State University
