Dear All,
So there has been quite a bit of advice on minimising radiation
damage, and on some of the effects of radiation damage, but unless I have
missed it no-one has come up with a clear cut case where radiation damage
actually resulted in the (complete ?) loss of a metal ion.
This makes Felix's question all the more relevant: In this particular case, is
there any certainty that the metal was there in the first place ? I know, for
example, that the catalytic metals are often missing in the binary complexes of
polymerases (protein + oligo but no incoming NTP), but this is not due to
radiation damage. Equally, in F1-ATPase we sometimes have a nucleotide bound
without the coordinative Mg ion.
I realise that Dean may not be able to give full details as this may be
commercially sensitive.
Best wishes,
Andrew
On 30 Apr 2014, at 11:33, Dean Derbyshire <[email protected]> wrote:
> Hi all,
> Has anyone experienced catalytic metal ions disappearing during data
> collection ?
> If so, is there a way of preventing it?
> D.
>
> Dean Derbyshire
> Senior Research Scientist
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