Thank you all for your reply! Here I summarize what I got from the answers:
1. Seems single channel pipette is a better choice than multi-channel with small volumn. 2. Positive displacement pipette is more advantageous than regular air displacement pipette. 3. Tips with repellent surface is also helpful. FYI, following are what I got recommended: Gilson microman (positive displacement, but the minimum volume is 1ul) http://www.gilson.com/Pipette/Products/44.224/Default.aspx#.U5Yqx5SSyG8 Hamilton syringe in a PB-600 clicker (need to work with Gastight syringe together) http://www.hamiltoncompany.com/products/syringes/c/211/ PICUS pipette (both single and multi channel has small dispensable volume <1ul) http://www.biohit.com/us/liquid-handling/pipettes-electronic/products/70/picus-electronic-pipette Eppendorf Repeater pipette OR just a regular Eppendorf 2.5ul pipette seems ok http://www.sigmaaldrich.com/catalog/product/aldrich/z683779?lang=en®ion= Rainin 20 uL single channel electronic repeating pipette (Need to refill every 20ul, but can dispense 0.5ul consistently). All of them are great, I think currently a repeating pipette or Hamilton syringe is just good enough for my purpose. I'll discuss with my colleague to decide which model we are gonna purchase. Again thank you all for your suggestion! Best, Xiao 2014-06-09 14:41 GMT-07:00 Xiao Xiao <[email protected]>: > Dear all, > > Here's a very basic question -- > I am doing some initial crystallization screening now but unfortunately > our robot got broken and it will take a while to get funded for buying a > new one:( So I have to set up all trays by hand. While it is still durable > to use our current pipettes sets, which are usually fine to dispense > 0.7ul-1ul per drop (not very accurate I think), I am wondering do you have > any recommended pipettes with smaller volume that meet my requirements? > > We are using Axygen 96-well sitting-drop plates for initially screening. > For the mother liquor I usually use regular multichannel pipette and it > works well, so I am seeking the one for the drop: > > 1. Multi-channel pipette that can accurately dispense about 0.7-1ul drop. > 2. Single pipette that can accurately dispense smaller drop size, such as > 0.4ul to 0.8ul. > > Thank you! > Best, > Xiao > > >
