Dear Appu, If it is possible to solve your structure with currently available MR models using any currently existing technology, one of these programs will be able to do it. Throwing yet another program at the problem won’t help, especially an older program that was good in its time (and is still, to be fair, cutting edge for some things) but is not up-to-date with current molecular replacement algorithms.
Some structures can’t be solved by molecular replacement. We can now do a better job of predicting which ones will be very hard, and this is given in the expected LLG output of Phaser. If that says that the problem is expected to be very difficult, you haven’t made any mistakes in running the programs, and you’ve tried all the strategies recommended by the authors of those programs, then your efforts will be better spent in trying to grow better crystals or doing experimental phasing. Best wishes, Randy Read On 12 Jun 2014, at 20:14, Appu kumar <[email protected]> wrote: > Hello All, > I have tried MR in Phaser, MRage, Molrep , Mrbump but i am > not getting the true solution which it supposed to be. Although the resoution > of data is 6.1A, but i want to give a try to CNS and see if I can find right > solution. I have read the manual of CNS but i am unable to get the CNS > running. Would you please help me in running the CNS program. > > Thank you > Appu ------ Randy J. Read Department of Haematology, University of Cambridge Cambridge Institute for Medical Research Tel: + 44 1223 336500 Wellcome Trust/MRC Building Fax: + 44 1223 336827 Hills Road E-mail: [email protected] Cambridge CB2 0XY, U.K. www-structmed.cimr.cam.ac.uk
