Dear all i have a small query to ask and seek your suggestions: I have collected a data for a protein with 324 residues and processed at its best in P212121. So Matthews suggest 1 mol in ASU with expected Mol. weight of 43 kDa with sovent content of 58% and 2 mol./ASU with 18% solvent content. However the data suggest possibility of translational NCS so i think i should ask for two molecules so that both get corrected for NCS. However for 2 mol./ASU, Matthewssuggests a total mol. weight of 52 kDa. So how to decide which way to proceed for MR?
Thanks Monica
