Dear all,
I have solved a structure (using molecular replacement) of
metallo-enzyme which may have Zn or Ni at its active site. I collected
data at in-house CuKa radiation. Now, I am able to locate the active site
metal ion preciously but I am not able to differentiate whether it is Zn or
Ni. I computed anomalous difference map and I got good anomalous map also.
But still there is ambiguity, since Zn and Ni have closest F" values at
CuKa radiation ( For Zn = 0.68 and For Ni = 0.51). But both, Zn and Ni have
different F' values at CuKa radiation ( For Zn = -1.61 and For Ni = -3.07).
My question is that,
*Can we used F' value information to differentiate metal ions?.Is it
possible to find whether I have Zn or Ni at active site of my enzyme using
crystallographic technique?. *
Thanks in advance
--
*Dhanasekaran Varudharasu*
Post-Doctoral Fellow
Department of Oral Biology
Rutgers school of Dental Medicine
Rutgers Biomedical and Health Sciences
Newark, NJ 07103
USA
