Of course, if you can plan ahead, you can grow your crystals in shipping friendly plates. See the In-Situ plate at Mitegen for details. http://www.mitegen.com/mic_catalog.php?c=insituplates
you also might try to add agarose to the drop to fix the crystal in place, but that could be tough with a very large drop (>20uL). Good luck! Bryan On Jul 2, 2014, at 5:33 PM, Sampson, Jared wrote: Hi Meisam - We do this frequently, using pieces of spongy foam to pad the inside of a sturdy styrofoam shipping box, which keeps the temperature from fluctuating too much. This is then carried in a reusable grocery bag by one person whose sole responsibility it is to ensure it isn’t subjected to any jarring movements during the trip. We haven’t used anything quite so large as your 10+ μl drops, but this setup works fine with 24-well plates of ~2-4 μl hanging drops for the 1.5-hour drive to our friendly neighborhood synchrotron. Cheers, Jared -- Jared Sampson Xiangpeng Kong Lab NYU Langone Medical Center http://kong.med.nyu.edu/ On Jul 2, 2014, at 4:17 PM, Meisam Nosrati <[email protected]<mailto:[email protected]>> wrote: Dear CCP4ers I need to transfer some crystals mainly in sitting drops to the site of data collection without freezing them. I do not know what is the best solution to secure the boxes in their place to minimize the disturbance. I am using the 24 well VDX plates with 10-80 microliter sitting drops. I have one or two hanging drop boxes as well with 10 microliter size drops. If you have any experience about this matter, I greatly appreciate, if you share it with me. Thanks Meisam Nosrati ------------------------------------------------------------ This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email. ================================= -------------------------------------------------------------------------- Confidentiality Notice: This message is private and may contain confidential and proprietary information. If you have received this message in error, please notify us and remove it from your system and note that you must not copy, distribute or take any action in reliance on it. Any unauthorized use or disclosure of the contents of this message is not permitted and may be unlawful.
