Dear all,
I'm trying to use Ample from CCP4 (6.4) to solve a structure of 2x197
residues from 2.0 A data.
After the generation of initial (120) models by Rosetta (3.3) Ample
stops with the error-message below.
Could anyone provide me information about the origin of this error and
possibly how to solve the problem?
Thanks in advance for your replies!
Best wishes,
Florian
***************************************************************************
* Information from CCP4Interface script
***************************************************************************
The program run with command:
/home/flo/crystallography/ccp4-6.4.0/bin/ccp4-python -u
/home/flo/crystallography/ccp4-6.4.0/bin/ample -mtz
/home/flo/crystallography/Virchow/ESRF_20140707/CM133A/ccp4/pos5wedge3scaled.mtz
-fasta /home/flo/Desktop/arch.seq -name MVD0 -run_dir
/home/flo/crystallography/Virchow/ESRF_20140707/pos7au/ccp4 -nproc 3 -F
F_XDSdataset -SIGF SIGF_XDSdataset -FREE FreeR_flag -models_dir
/home/flo/crystallography/Virchow/ESRF_20140707/pos7au/ccp4/ROSETTA_MR_0/models/models_1
-early_terminate True -use_shelxe True -use_arpwarp False
has failed with error message
Traceback (most recent call last):
File "/home/flo/crystallography/ccp4-6.4.0/bin/ample", line 757, in
ensemble.create_ensembles( amopt.d )
File "/home/flo/crystallography/ccp4-6.4.0/share/ample/python/ensemble.py",
line 85, in create_ensembles
ensembles = ensemble_models( amoptd['spicker_results'][ cluster-1
].pdb_list, amoptd, ensemble_id=cluster )
File "/home/flo/crystallography/ccp4-6.4.0/share/ample/python/ensemble.py",
line 58, in ensemble_models
percent=amoptd['percent'] )
File
"/home/flo/crystallography/ccp4-6.4.0/share/ample/python/ample_ensemble.py",
line 168, in generate_ensembles
self.make_truncated_ensembles()
File
"/home/flo/crystallography/ccp4-6.4.0/share/ample/python/ample_ensemble.py",
line 312, in make_truncated_ensembles
clusterer.generate_distance_matrix( self.truncated_models[tcount] )
File
"/home/flo/crystallography/ccp4-6.4.0/share/ample/python/cluster_with_MAX.py",
line 55, in generate_distance_matrix
retcode = ample_util.run_command( cmd, logfile=log_name )
File "/home/flo/crystallography/ccp4-6.4.0/share/ample/python/ample_util.py",
line 274, in run_command
p = subprocess.Popen( cmd, stdin=stdin, stdout=logf,
stderr=subprocess.STDOUT, cwd=directory, **kwargs )
File "/home/flo/crystallography/ccp4-6.4.0/lib/python2.7/subprocess.py", line
711, in __init__
errread, errwrite)
File "/home/flo/crystallography/ccp4-6.4.0/lib/python2.7/subprocess.py", line
1308, in _execute_child
raise child_exception
OSError: [Errno 8] Exec format error
***************************************************************************
--
Dr. Florian Sauer
Rudolf Virchow Zentrum für Experimentelle Biomedizin
Josef-Schneider-Str. 2, Haus D15
97080 Würzburg
