Hi Sajid, *Assuming* you have one site per monomer (rather than, say, one site per dimer), and *assuming* each binding event is completely independent ( I.e no co-operativity), you might just get away with running the experiment with the heterogeneous material.
However, you might not be able to confidently make these assumptions, so imho it would be preferable to separate the monomer and dimer by SEC prior to ITC. If this is not possible, then pay close attention to the fit when you run the heterogeneous experiment. Poor fit to a one site model may indicate that these assumptions are invalid. Can you obtain stoichiometry information from a different technique? This might be very helpful. Hth, Dave Dr David C Briggs PhD http://about.me/david_briggs On 18 Jul 2014 10:25, "sajid akthar" <b_sajid_...@yahoo.co.in> wrote: > Dear All, > > This is an off-topic question. I have protein solution of heterogeneous > (contains both monomer and dimer). I want to perform ITC with this protein. > I doubt whether this heterogeneity will interfere the binding study. > > Any advice please. > > Thank you > > Sajid >
