By all means, try it both ways and see whether the R-Rfree gap narrows with random vs thin shell selection. Depending on resolution and data quality, you may also consider imposing NCS restraints.
Sent on a Sprint Samsung Galaxy S® III <div>-------- Original message --------</div><div>From: Xianchi Dong <[email protected]> </div><div>Date:08/12/2014 4:45 PM (GMT-05:00) </div><div>To: [email protected] </div><div>Subject: [ccp4bb] thin shell Rfree set selection </div><div> </div>Dear all, I have a dataset of C2 symmetry and 2 molecules in the ASU. I am wondering if I have to use thin shell Rfree set selection to avoid Rfree bias by NCS. And which Rfree selection method is better? Thanks in advance. Xianchi
