Dear Avinash,
you can use Ellman's reagent (DTNB) for GSH.
Have a look here:
http://strucbio.biologie.uni-konstanz.de/ccp4wiki/index.php/Thiols_and_disulfides
For glutathione you don't have to use a buffer with GuHCl.
HTH, Guenter
Dear All,
Greetings and apology for an off topic query. may request you all to
kindly share with me a conventional protocol, by which i can calculate
a total Glutathione content (oxidized + reduced) in an
/in-vitro/ reaction set up.
all i find over internet are kit-based methods, which turns out to be
quite expensive for couple of reactions i would like to do.
thanks in advance
kind regards
Avinash Kale
