Dear Rana, Brad's post compelled me to reply to you. The Cryo EM field is well into its pro stage.
If you can be specific about the MolWt, size and if it makes any complex, then much precise and helpful answers to your question can be provided. Best Anindito Sen Ph.D JeoL > On 29/08/2014, at 11:31 pm, Marcus Fislage <[email protected]> wrote: > > Hi Rana, > > in contrast to Brads answer, and in case I understand you well, Cryo EM is > not in ts infancy stage (as long as you do not talk about Cryo EM on crystals. > For cryo EM you need only several ul of a nM solution. > I can always suggest you the 3dem bb. There are of course still limitataions > on the size of protein target and resolution, but those are slowly falling. > > Some reading material > http://www.ncbi.nlm.nih.gov/pubmed/23181775 > http://www.ncbi.nlm.nih.gov/pubmed/25071206 > > > Cheers > Marcus > > Quoting Brad Bennett <[email protected]>: > >> Yes, but the technique is still in its infancy stage. >> >> DOI: 10.1016/j.sbi.2014.03.004 >> >> Cheers- >> Brad >> >> >> On Fri, Aug 29, 2014 at 6:34 AM, rana ibd < >> [email protected]> wrote: >> >>> Dear CCP4 >>> I wanted to ask has anyone tested 3D protein structure by cryo-electron >>> microscopy? what is the suitable concentration required for this procedure >>> Best Regards >>> Rana > > > > -- > Marcus Fislage, PhD > > Howard Hughes Medical Institute (HHMI) > Columbia University > Department of Biochemistry and Biophysics > Lab of Joachim Frank > New York, NY > > Phone: 212.305.9524 > Fax: 212.305.9500
