The request to calculate a map using DANO means the CCP4 implemtation of the FFT program moves the input PHIC phase by 90 degrees as Zbysek says.. Eleanor
On 29 August 2014 20:23, Pavel Afonine <[email protected]> wrote: > See also: > > page 141 in "The Bijvoet-Difference Fourier Synthesis", > Jeffrey Roach, METHODS IN ENZYMOLOGY, VOL. 374 > > Pavel > > > On Fri, Aug 29, 2014 at 12:17 PM, Ronald E Stenkamp < > [email protected]> wrote: > >> I think the answer is in this paper: LOW-RESOLUTION ELECTRON-DENSITY AND >> ANOMALOUS-SCATTERING-DENSITY MAPS OF CHROMATIUM HIGH-POTENTIAL IRON PROTEIN >> By: STRAHS, G; KRAUT, J >> JOURNAL OF MOLECULAR BIOLOGY Volume: 35 Issue: 3 Pages: 503-& >> Published: 1968 >> >> >> >> >> On Fri, 29 Aug 2014, Alexander Aleshin wrote: >> >> Could anyone remind me how to calculate anomalous difference Fourier >>> maps using model-calculated phases? I was doing it by >>> (1) calculating PHcalc from a pdb file using Sfall, then >>> (2) merging PHcalc with Dano of experimental SFs, then >>> (3) calculating a map with Dano and PHcalc using FFT program of CCP4. >>> >>> Now, I've read Z. Dauter's et all paper http://mcl1.ncifcrf.gov/ >>> dauter_pubs/175.pdf, and it said that their anomalous maps were >>> calculated using (delF, PHcalc-90degrees). Why did they use -90 degrees? >>> How does it relay to a (delF, phcalc) map? >>> >>> Thank you for an advice. >>> >>> Alex Aleshin >>> >> >
