Hi, All CCP4BB Users,

I have quite some data sets(~15) collected at different beam intensities,
the individual dataset can diffract to  ~3.8 A @I/dI=2. If I combine them,
with AIMLESS in CCP4, the resolution can be extended to ~3A@I/dI=2. But,
the Rmerge or Rpim is way high, in 10s or even 100s. Would this high R
simply due to the intensity difference among these datasets? Or simply say,
can I trust/use the combined/merged dataset diffracting to 3A?

Thanks!

Charles

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Charles Chen

Research Associate

University of Pittsburgh School of Medicine

Department of Anesthesiology

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