On 04/09/14 19:06, Lie Ma wrote:
Hello all,

I am trying to make a model of an oligosaccharide which contains several sugar 
monomers. I obtained the monomers from coot library and put them into the 
electron density. But I don't know how to make covalent bond between these 
monomers.

If you are using Coot, you can use Extensions -> Modelling -> Make Link.

If you sugar monomers are reasonably placed Coot's Sphere Refine will determine the correct link types on the fly (it doesn't believe what's in the PDB file).


I tried to use PRODRG to generate a model and it gave me one big ligand 
consisting of several monomers.

Yes, that's not the way to do it...

  However, what I want is to have a chain contains several monomers, and each 
monomers acts like a residue.

Indeed.


Paul.

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