Hi,
a while... especially in powder form, but even in liquid.
The easiest is to test if they are still "active"/working. For the ones
that covalently bind, you can check a difference in migration on
native-PAGE.
If it doesn't work, you can check SDS-PAGE and FD-HAL (Fluorescence
Detection of Heavy Atom Labeling), on the ones that bind to CYS.
It's always good to test by biochemistry the good labelling atoms before
going into soaking, prelabelling, etc...
Hope it helps.
Vincent
Le 9/22/14 5:33 PM, Shanti Pal Gangwar a écrit :
Dear all
For what time period (years) heavy atom screens from Hampton research
remain active/functional if kept in lyophilsed state or get expired
over time.
Thanks in advance
Shanti Pal
********************
Shanti Pal Gangwar, Ph.D
Dept of Pharmacology and Toxicology
University of Texas Medical Branch
Galveston, Texas-77555
Email: gangwar...@gmail.com <mailto:email%3agangwar...@gmail.com>
--
Vincent Chaptal, PhD
Institut de Biologie et Chimie des Protéines
Drug-resistance modulation and mechanism Laboratory
7 passage du Vercors
69007 LYON
FRANCE
+33 4 37 65 29 01
http://www.ibcp.fr
