Hi Manjula,

I imagine that they could be reusable, if you stored them in a buffer with
some sort of preservative like sodium azide (NB Toxic) or maybe a
bacteriostatic agent like EDTA or something.

However, when you use them, a small amount of protein is going to remain in
the cassette after each use, so I would only use each cassette for a
specific protein to prevent cross-contamination. Additionally, I'd worry
about protein from old preps falling apart/denaturing and then
contaminating future fresh preps of protein. I guess it depends at what
step during purification you are using them.

HTH,

Dave



[image: David Briggs on about.me]

David Briggs
about.me/david_briggs
  <http://about.me/david_briggs>

On 26 September 2014 15:51, Manjula Ramu <[email protected]> wrote:

> Hi all,
>
> I use Slide-A-Lyzer MINI Dialysis Devices from Thermo for dialysis of
> small protein volumes. Are they reusable? if so how can we store them??
>
> Thanks and Regards,
> Manjula R
> Research Scholar
> Department of Biophysics
> National Institute of Mental Health and Neurosciences
> Bengaluru-29, Karnataka
> INDIA
> E-mail: [email protected]
> Mobile no:+91-9538553356
> http://www.nimhans.kar.nic.in/
> <https://www.nimhans.kar.nic.in/>
>

Reply via email to