Dear Manjula, Unless Thrombin requires BSA for functioning (I never used Thrombin) you could run a size exclusion column with the Thrombin solution prior to using it. Or you could by it from a different vendor.
Regards, Tim On 10/08/2014 03:27 PM, Manjula Ramu wrote: > Hi all, > > My protein size is nearly the same size of BSA and it has His tag. If I > have to cleave the tag I have to use Thrombin which is having huge amount > of BSA in it. Now how will I separate this BSA from my protein. > > Thanks and Regards, > Manjula R > Research Scholar > Department of Biophysics > National Institute of Mental Health and Neurosciences > Bengaluru-29, Karnataka > INDIA > E-mail: manjula....@gmail.com > Mobile no:+91-9538553356 > http://www.nimhans.kar.nic.in/ > <https://www.nimhans.kar.nic.in/> > -- Dr Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen GPG Key ID = A46BEE1A
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