Dear, the conditions you list should equilibrate within a couple of days. A growth time of three months could be a sign of some proteolytic reaction happening. You might want to sequence the crystal and subclone the fragment that actually crystallises.
Best, Tim On 10/31/2014 11:05 AM, Vijaykumar Pillalamarri wrote: > Dear all, > > I am trying to crystallize a 30 kD protein. Protein crystals are formed > after 3 months. The crystals are formed in the following condition: > 0.01M Zinc sulphate > 0.1M MES monohydrate pH 6.5 > 25% v/v PEG monomethyl ether 550 > > Please suggest me how to grow these crystals faster. > > Thanking you > -- Dr Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen GPG Key ID = A46BEE1A
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