How appropriate that someone whose name is Picot would be
the first one to have the answer.

If you don't get it, put define:picot in a google search box.

                      Frances

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On Mon, 3 Nov 2014, Daniel Picot wrote:

Hi Phhilippe,

T. Y. Teng, J. Appl. Crystallogr.
23,
J. Appl. Crystallography 23:387?391 (1990)
Mounting of crystals for macromolecular crystallography in a free-standing thin 
film

A method for mounting single crystals in macromolecular crystallographic 
studies is described
in which the crystal is suspended in a thin film. The film is formed from a 
mixture of the
crystallization buffer and a hydrophilic viscous material, confined within a 
thin-wire loop by
surface tension. Compared with conventional crystal mounting methods, this 
method greatly
simplifies and speeds the mounting procedure, is well suited to shock freezing 
and to optical
monitoring of the crystals, deforms fragile crystals less and gives a lower and 
more uniform
background in the X-ray diffraction patterns.

I do not have a copy, but if I remember well it was done with a copper wire, 
hair and nylon
loops came later.
Daniel



Le 03/11/2014 18:23, DUMAS Philippe (VIE) a ?crit :

I am looking for the reference of this little paper (in J. Appl. Cryst ?) 
describing how to mak
e a loop and fish crystals...
You know, this kind of humble method used by all crystallographers (apart FEL 
users).
Thank you in advance

Philippe Dumas



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