Hi Sasha,
this has been discussed on this list in the past, on several occasions.
If a consensus has been achieved, I've missed that.
For what it's worth, I don't see why you'd remove a side chain or set
the occupancy to 0 without experimental evidence for its absence or
non-occupancy. I let a high B value indicate its flexibility.
Andreas
On 11/11/2014 1:14, Sasha Pausch wrote:
Dear CCP4bb,
Sorry for asking a naive question.
I am trying to deposit a structure in PDB. I would like to know whether
we have to delete the side chains of amino acids for which we are not
finding density or people prefer keeping the side chains occupancy zero?
Is there any other way to do this?
--
Andreas Förster
X-ray Crystallography Facility Manager
Centre for Structural Biology
Imperial College London
