Hi Jeorge, The simplest answer is that you have multiple positions for the Methionine. So you can try adding in an alternate position for it. However, you didn’t mention the sigma cutoff or e-/A^3 for either of your density maps. Its quite possible that your fofc map is just scaled way down and what you are seeing is an exaggeration of what’s barely there. Hope this helps. Cheers!
- Greg ------------------------------------------------------------------------------- Greg Costakes, Ph.D. Department of Biochemistry University of Cambridge 80 Tennis Court Road Cambridge, CB2 1GA United Kingdom -------------------------------------------------------------------------------- From: jeorgemarley thomas Sent: Wednesday, November 12, 2014 8:16 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Refinement with refmac05 and methionine density Dear All, I am refining the structure with refmac05, and after each refinement the density around this methionine is flipping. and it is difficult to say where this methionine actually have the density. please suggest what I should I do. I am attaching the snap here. Thanks in Advance for your kind suggestions Jeorge --- This email is free from viruses and malware because avast! Antivirus protection is active. http://www.avast.com
