Dear Crystallographers,

I thought that for reliable values for Rfree, one needs only to satisfy 
counting statistics, and therefore using at most a couple thousand reflections 
should always be sufficient. Almost always, however, some seemingly-arbitrary 
percentage of reflections is used, say 5%. Is there any rationale for using a 
percentage rather than some absolute number like 1000?

All the best,

Jacob

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Jacob Pearson Keller, PhD
Looger Lab/HHMI Janelia Research Campus
19700 Helix Dr, Ashburn, VA 20147
email: [email protected]
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