Hi everyone !! I am working on a protein which binds to its ligand in a particular state i.e. Fe3+ state (active) and reduces it to Fe2+ state (inactive). I am trying to set up crystallisation trials with the ligand but seems like difficult to get them. However i just needed an advice here, is there any way to know whether the protein is in Fe2+ state or Fe3+ state or how can i make the protein to exist in more of Fe3+ state so that i increase the probability of ligand binding to the protein.
Your advice is highly appreciated. Thanks in advance !! Cheers Monica
