Hi Mark, Thanks for your suggestion. I have already got the solution from Phaser. Just wanted to know the problem with Balbes, why it is taking random structures as template?
regards Atul On Thu, Nov 27, 2014 at 8:34 PM, Mark J van Raaij <[email protected]> wrote: > Just use Amore, Molrep or Phaser and give them the apo structure as model. > I especially recommend Amore for first-time MRers, as it forces you to > understand some basics of MR. And it runs very fast. > Molrep and especially Phaser can probably solve more difficult problems > with lower homology models and are easier to use than Amore, but usually > take a bit longer. > Balbes is overkill for a problem like this. > > Mark J van Raaij > Lab 20B > Dpto de Estructura de Macromoleculas > Centro Nacional de Biotecnologia - CSIC > c/Darwin 3 > E-28049 Madrid, Spain > tel. (+34) 91 585 4616 > http://www.cnb.csic.es/~mjvanraaij > > > > > > > > On 27 Nov 2014, at 21:19, Atul Kumar wrote: > > > Hi all, > > > > I am trying to solve complex structure of my proteins. Apo protein > structure is already known. Balbes should take this structure as template > for MR, whereas, it is taking some random proteins as template. I was > wondering, whether it is synchronized with pdb or uses its own databank. > Since structure of this protein deposited in pdb last year, wondering > whether or not it is updated in its local databank, in case it is not > synchronized with pdb. Can somebody help to figure out this problem? > > > > regards > > > > Atul > >
