Are you testing all SGs? for your known point group?

Eleanor

On 28 November 2014 at 13:34, rohit kumar <[email protected]> wrote:

>
>
> Dear all,
>
> i am solving a structure of 2.5 A resolution by PHASER but every time i
> got this following warning.
>
> $TEXT:Warning: $$ Baubles Markup $$
>
> -------------------------------------------------------------------------------------
>
> Large non-origin Patterson peak indicates that translational NCS is present:
>
> correction factors applied
>
> -------------------------------------------------------------------------------------
>
> $$
>
> $TEXT:Warning: $$ Baubles Markup $$
>
> -------------------------------------------------------------------------------------
>
> Solutions with Z-scores greater than 49.2 (the threshold indicating a definite
>
> solution) were rejected for failing packing test
>
> #1 TFZ=65.6 PAK=42
>
> #2 TFZ=65.5 PAK=68
>
> -------------------------------------------------------------------------------------
>
> however every time PHASER run successfully and it give a output PDB as a 
> solution. is it a right solution or not?  or To get the right solution what 
> should i do?
>
>
> if i used accept all the solution in expert parameter, i also got the 
> solution but which does not follow the Packing test.
>
>
> Can anyone suggest...
>
>
> --
> WITH REGARDS
> Rohit Kumar Singh
> Lab. no. 430,
> P.I. Dr. S. Gourinath,
> School of Life Sciences,
> Jawaharlal Nehru University
> New Delhi -110067
>
>
>
>
>

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