Dear Connie,

pymol uses the APBS program to calculate the ESP, which in turn needs a
'pqr'-file as input. This pqr-file contains all atoms with the entries
for the B-value replaced with partial charges. It is probably better to
compare these partial charges than the redness of the surfaces.

Best,
Tim

On 11/30/2014 09:26 PM, Constance Jeffery wrote:
> Dear CCP4 users,
> I have a question about protein electrostatic surfaces.  We used Pymol to 
> calculate electrostatic surfaces of several protein structures.  Is there a 
> way to calculate the percent of surface that is red, or the area of surface 
> that is red, so that we can quantitatively compare these structures?
> 
> Thanks,
> Connie 
> 

-- 
Dr Tim Gruene
Institut fuer anorganische Chemie
Tammannstr. 4
D-37077 Goettingen

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