Hi Reza, 1.0 and 1.25 M citrate are potential cryo solutions. They still had faint ice rings present but were not dominant. It is also difficult to make a solution more concentrated then 1.75 M citrate. Hope this helps.
-Adam > On Dec 1, 2014, at 10:59 AM, Reza Khayat <[email protected]> wrote: > > Hi, > > Has anyone used citrate as the sole cryoprotectant? If so, > what concentration was needed? > > Best wishes, > Reza > > Reza Khayat, PhD > Assistant Professor > The City College of New York > Department of Chemistry, MR-1135 > 160 Convent Avenue > New York, NY 10031 > Tel. (212) 650-6070 > www.khayatlab.org
