Practical X-ray course on methods in protein crystallography: data collection, data processing, and phasing (May 18 till May 22, Oulu, Finland) There will be an international X-ray course at the Faculty of Biochemistry and Molecular Medicine and the Biocenter Oulu, of the University of Oulu, Oulu, Finland. The course will focus on data collection and data processing using iMOSFLM and XDS, to be complemented by lectures and tutorials on experimental phasing (SHELX) and molecular replacement phasing (CCP4). In addition there will be lectures on new techniques (XFEL diffraction and neutron crystallography). On the last day of the course there will be a remote data collection demonstration at Diamond Light Source, Oxford. The outline of the course follows the previous format, aiming for approximately 20 students, who will be provided with a computer in our linux class room. Data collection from own crystals will be possible using our in-house Bruker microfocus X-ray generator or remotely at Diamond. Throughout the course students will have time to work on their own topics in the linux class room and we will also provide the opportunity to practice with cryocooling of own crystals and collect data with our in-house equipment. Crystals that should be used for data collection in house or at Diamond should be available for freezing on the first day of the course or should be sent in advance to Oulu. The accommodation for non-local students will be arranged by the local organisers. Please contact Lari Lehti? (lari.lehtio (at) oulu.fi) or Kristian Koski (kristian.koski (at) oulu.fi) for further information regarding the course. When applying to attend this course follow the instructions below. Website for the course: <http://www.oulu.fi/biocenter/instruct-nac/courses> http://www.oulu.fi/biocenter/instruct-nac/courses Current course outline: Monday, May 18: Basic concepts of biomolecular crystallography, by Bernhard Rupp (Insbruck, Austria and Vista, USA) Emerging techniques: XFEL by Janos Hajdu (Uppsala, Sweden): "The basic principles of the X-ray free electron laser technology and molecular imaging" Afternoon tutorial by Bernhard Rupp on "Refinement, validation, COOT, PDB, PDB_REDO" Afternoon lecture By Manfred Weiss on the X-ray beamlines at BESSY, Berlin, Germany Tuesday, May 19 (dataprocessing): XDS, Manfred Weiss (BESSY, Berlin, Germany) (also XDSapp as available at Bessy) iMOSFLM, Harry Powell (LMB, Cambridge, UK) (also data processing pipelines at Diamond) Tuesday-afternoon tutorial on XDS (Weiss) Wednesday, May 20 (phasing): Experimental phasing by SHELX, Thomas Schneider (EMBL, Hamburg, Germany) Molecular replacement phasing by CCP4, Andrey Lebedev (CCP4, STFC, UK) Wednesday-afternoon: tutorial on iMOSFLM (Powell) Wednesday afternoon lecture on neutron crystallography by Dr. Zoe Fisher (ESS, Lund, Sweden) Thursday, May 21: Thursday morning lecture by Thomas Schneider on the X-ray beamlines at EMBL, Hamburg Thursday morning: tutorial on SHELX (Thomas Schneider) Thursday afternoon: tutorial on Molecular replacement (Andrey Lebedev) Friday, May22 (remote datacollection): Remote data collection at Diamond (Martin Walsh, Diamond, UK) Good practice for preparing data collection at synchrotrons (Kristian Koski, FBMM, University of Oulu, Finland) Crystal sample management and data tracking from crystal to PDB by xtalPiMS (Ed Daniel, FBMM, University of Oulu, Oulu, Finland) Application instructions In order to apply to the course please apply by sending the following documents to Lari Lehti? (lari.lehtio (at) oulu.fi) by 15.01.2015 The applications will be reviewed and all the participants will be informed about the decision in the end of January 2015. Include in your application a pdf of 1. your CV 2. A motivation letter describing prior experience and why the course would be beneficial for you (please include if you will provide crystals and or datasets) 3. A support letter from your supervisor Name all the documents similarly: surname_motivation_letter.pdf etc. For the email use a subject line: Application for the X-ray course Best regards, Lari Lehti?/Kristian Koski/Tiila Kiema/Rik Wierenga
