Re: [ccp4bb] ITC thermogram

[Atul Kumar]

Probably, you have two mode binding between your ligand and protein. First
time, when you had approximately 7 times molar excess of ligand, you could
not monitor the endothermic reaction. When you went down to approx 2.5x
lower conc of ligand, you could monitor endothermic reaction. However, what
is Kd of the reaction? Is it very strong binding? How much micro cal values
you are getting? When you dont see saturation, you increase the conc of
ligand, why did you go down?