The suggestions made are good ones. Honestly, each crystal necessitates its own cryo screening, this is part of the job of the crystallographer in the cryo age. A couple of my own suggestions:
1. try shooting some crystals at room temperature to get a baseline diffraction before trying different cryos (I.e. “do my crystals diffract at all, or are the cryos I’m trying ruining the crystal?”) 2. I’ve had good luck bypassing the cryo screening route altogether by embedding crystals into paratone-N oil. This takes some practice maneuvering crystals out of the aqueous phase into the viscous oil, but I really like this strategy. I recommend practicing on some of your not-so-good crystals or dummy crystals of lysozyme to get the technique down. Good luck! Geoff - Geoffrey K. Feld, Ph.D. IRTA Fellow Genome Integrity and Structural Biology Laboratory National Institute of Environmental Health Sciences 111 T.W. Alexander Drive Building 101, Room F-331 Research Triangle Park, NC 27709
