How about “whether the structure can be solved” as a criterion, i.e, maybe just 
put it into your favorite software and see whether it works, trying various 
parameters? Depends on your goals, I guess―most people just want to solve the 
structure and move on.

JPK

From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of luzuok
Sent: Friday, January 16, 2015 9:33 AM
To: [email protected]
Subject: [ccp4bb] Assess anomalous signal?


Dear all,
    I'm doing Hg SAD phasing for the first time, and I met some problem:
1. How to assess the anomalous signal after I process the data and get my mtz 
file?
    My labmate doesn't scan the fluorescence signal. Actually, I don't quite 
understand why we use fluorescence to detect anomalous signal.
2. How to estimate the number of heave atom in one unit cell? by soaking heavy 
atom derivatives.

Best!

Lu Zuokun
Nankai University

--
卢作�j
南开大学新生物站A202

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